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. 2015 Jan 14;4(2):197–205. doi: 10.1242/bio.201410629

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — MDCK cells transiently transfected with prestin YFP, and the mutations Y520Q and Y667Q contained in the prestin YFP cassette were fixed after 12, 20, 30 and 40 hours after transfection. The cells were then stained with antibodies to the Golgi protein Giantin. While prestin YFP exits the Golgi at 20 hours, Y520 dos not egress from the Golgi and Y667Q leaves the Golgi after 40 hours. A graphical form of Pearson's correlation between prestin YFP and Giantin at the different time points is shown below. For wt prestin there was a significant difference on one way ANOVA between the Pearson's correlation between prestin and Giantin at all times compared to 12 hours: 12 hours vs 20 hours, P<0.05; 12 hours vs 30 hours, P<0.001; and 12 hours vs 40 hours P<0.001. In contrast, the differences in Pearson's correlation between Y520Q and Giantin at all times compared to 12 hours were not significantly different (P>0.05 on one way ANOVA). For Y667Q the Pearson's correlation with Giantin was significantly different on one way ANOVA between 12 hours and 40 hours (P<0.05), while the remaining two time points were not significantly different (12 hours vs 20 hours, P>0.05; 12 hours vs 30 hours, P>0.05). The mean values for the different time points were as follows: wt prestin 12, 20, 30 and 40 hours: 0.23 (+/−0.08 SE, n = 5), 0.09 (+/−0.04 SE, n = 10), −0.06 (+/−0.03 SE, n = 12), −0.162 (+/−0.02 SE, n = 13); Y520Q 12, 20, 30 and 40 hours: 0.17 (+/−0.02 SE, n = 5), 0.114 (+/−0.04 SE, n = 6), 0.145 (+/−0.06 SE, n = 13), 0.08 (+/−0.02 SE, n = 8); Y667Q 12, 20, 30 and 40 hours: 0.112 (0.03 SE, n = 13), 0.04 (+/−0.02 SE, n = 9); 0.026 (+/−0.05 SE, n = 8) and −0.04(+/−0.03 SE, n = 8). The scale bar is 10 microns.