Figure 1.

Detection of HOTAIR expression in tumor tissues and CD117 ⁺ CD44 ⁺ CSCs identified by qRT-PCR and FCM. A. The HOTAIR expression in four patient’s EOC tissues and six SKOV3 tumor tissues in the mice tested by qRT-PCR. B and C. The cell phenotype of CD44⁺CD117⁺cells sorted from the SKOV-3 cells by using the magnetic-activated cell sorting system (B) and the SKOV-3 cells (C) were analyzed by FCM, indicating 92.3% and 3.1%, respectively, of the double positive phenotypes of CD44 and CD117 in sorted cells and SKOV-3 cells. D. QRT-PCR analysis of the HOTAIR expression in both the CD117⁺CD44⁺ cells and the non CD117⁺CD44⁺ cells. **p < 0.01 and ***p < 0.001 were calculated by using the Student’s t test method, referring to the differences as indicated.