Figure 2.

HOTAIR-shRNA SKOV3 cells decreased the ability of cell migration and invasion. A-D. RNA and proteion extracts from 1 × 10⁶ SKOV3 cells were respectively subjected to qRT-PCR and Western blot assays for detection of the expression of HOTAIR (A) E-cadherin (B, D) and Vimentin (C, D) in the SKOV3 cells transfected with siHOTAIR or siCONTAL (scramble). E. In vitro wound healing, 5 × 10⁵ different cells were respectively plated in 6-well plates to form a monolayer, and on the following day, a uniform scratch was made down in the center of well using a sterile micropipette tip. Wound closure (light microscopy, ×200) is presented the percentage reduction of the freshly wounded area. F. Quantification of the wound healing assay result. G. The invasive assay result shows that 5 × 10⁵ different SKOV3 cells were seeded in the upper chamber in RPMI1640 with serum-free. Cells that invaded to the lower surface of the Matrigel-coated membranes after being incubatied for 48 h, next fixed with 70% ethanol, and finally stained with trypan blue. Cells from five randomly selected fields were counted under a light microscope (magnification ×100). H. Quantification of the invasive assay result. * P < 0.05 and **P < 0.01, referring to the differences as indicated.