Figure 3.

CD117 ⁺ CD44 ⁺-shHOTAIR reduced the HOTAIR expression and colony forming potential. A. CD117⁺CD44⁺ CSCs transfected with the recombinant shHOTAIR or scramble HOTAIR were selected by 800 μg/ml G 418 in two weeks (magnification 200×). Images were taken from a fluorescence microscope (top) and from a light microscope (bottom). B. QRT-PCR analysis of the HOTAIR expression in both CD117⁺CD44⁺-shHOTAIR and CD117⁺CD44⁺-scramble. C. Images of CD117⁺CD44⁺- shHOTAIR and CD117⁺CD44⁺-scramble in colony forming assay. D. Statistical analysis of colony forming ratio. **P < 0.01.