Figure 6.

In C/EBPα-expressing Hep3B cells TMEM166 was required for the induction of autophagy. (A) The TMEM166 protein was determined by western blotting in stable cell lines. (B-D) In Hep3B cells TMEM166 was silenced by specific siRNA before starvation. The sub-G₁ cell deaths were determined (B), western blotting is shown (C), and intracellular triglyceride levels were measured (D). (E,F) The TMEM166 protein was overexpressed in the low-expressing HCC-M cells and then starved. Abbreviations: FU, full medium; NG, glucose- and glutamine-free medium.