Table II.
Oligonucleotide primers used in this study
| Primera | Nucleotide sequence,b 5′–3′ | Construct |
|---|---|---|
| cps14B-ID1 | GACCAAGATAACATCACCGC | p4Δcps14B |
| cps14B-ID2 | GGTTGGCATTTGTCTACAGT | p4Δcps14B |
| cps14B-ID3 | ACCACTCCAAATACAAAACG | p4Δcps14B |
| cps14B-ID4 | GCTCGCGCTATATTCTCTTG | p4Δcps14B |
| cps14B-ID5 | CGACTTGGTGGGTGGAATTG | p4Δcps14B |
| cps14B-ID6 | TCTTCGATGTCCTGAGGACGGTCAACACTGCAGTTAAGAG | p4Δcps14B |
| cps14B-ID7 | CTCTTAACTGCAGTGTTGACCGTCCTCAGGACATCGAAGA | p4Δcps14B |
| cps14B-ID8 | GGTTTCTCCCAACCCTACTG | p4Δcps14B |
| neu14C-ID1 | CGGTGATGTTCATCTAGCACGG | p4Δneu14C |
| neu14C-ID2 | AGCGATCCCCCAGAATCAACAC | p4Δneu14C |
| neu14C-ID3 | CACAGCCGAAGAACAAACGCAG | p4Δneu14C |
| neu14C-ID4 | TGGACGCATGAGGACTTGAACC | p4Δneu14C |
| neu14C-ID5 | TCTCAGCTCGAAATGACTCGTC | p4Δneu14C |
| neu14C-ID6 | CATGGTTGAGGCCTGACGAGAGCCTGTCAC | p4Δneu14C |
| neu14C-ID7 | GTGACAGGCTCTCGTCAGGCCTCAACCATG | p4Δneu14C |
| neu14C-ID8 | AGGTCCCTGACTCCGTCAAC | p4Δneu14C |
| pCPS14BF_NcoI | AGCCATGGAGTCCGTACTTGTTTA | pMX14B |
| pCPS14BR_EcoRI | GTACGTGGAATTCCTAACATTGCC | pMX14B |
| pNEU14CF_PstI | TGAGCTGCAGCAAAATATTTGCCATAGTGC | pMXNEU14C |
| pNEU14CR_PstI | CATCTGCAGAGGTACCCGCTCCTAGAAAGG | pMXNEU14C |
a
Obtained from Integrated DNA Technologies, Coralville, Iowa, USA.
b
Restriction sites are underlined.