FIGURE 1.

Development of Hordeum sp. introgression lines in hexaploid wheat in the ph1b mutant background. Crosses between a Hordeum sp. substitution or addition line in bread wheat cv. Chinese Spring (2n = 6x = 42) and the ph1b mutant in hexaploid wheat were developed and backcrossed to the ph1b mutant to obtain Hordeum sp. introgressions in the absence of the Ph1 locus. Screening and characterization of chromosome complements were carried out by multicolor in situ hybridization and molecular markers analyses.