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. 2015 Mar 19;6:160. doi: 10.3389/fpls.2015.00160

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Copyright © 2015 Rey, Calderón and Prieto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Various forms of introgression (arrowed) detected by GISH (Hordeum chilense and H. vulgare genomic introgressions detected in green and red, respectively), and FISH patterns (GAA and pAs1 probes detected in red and green, respectively), in the BC₁F₂ progeny derived from the crosses Hordeum addition/substitution line × ph1b mutant. (A) GISH and (B) FISH pattern of chromosomes of a partial mitotic metaphase carrying two copies of a 4D chromosome with a distal 4H^ch L segment. (C) GISH and (D) FISH pattern of a mitotic metaphase carrying two copies of a 4H^ch chromosome with a distal 4D L segment. (E) GISH and (F) FISH pattern of a mitotic metaphase carrying a homocigous 7H^ch S–7A L Robertsonian translocation. (G) GISH and (H) FISH pattern of a mitotic metaphase carrying a homocigous 7A S–7H^ch L Robertsonian translocation. (I) GISH of a 4H^v monotelosomic line. (J) GISH of a 6H^v monotelosomic line. Bar: 10 μm.