Abstract
Binding of prothrombin and activation intermediates 1 and 2 to human platelets was tested with 125-I-labeled protein preparations. None of these precursors of thrombin bound to platelets under conditions in which high affinity binding of thrombin was observed,nor did they cause platelet aggregation or serotonin release. The molecular conformation required for binding to platelets as well as for induction of platelet aggregation and release is present, therefore, only after the final step in prothrombin activation.
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- Davey M. G., Lüscher E. F. Actions of thrombin and other coagulant and proteolytic enzymes on blood platelets. Nature. 1967 Dec 2;216(5118):857–858. doi: 10.1038/216857a0. [DOI] [PubMed] [Google Scholar]
- Esmon C. T., Jackson C. M. The conversion of prothrombin to thrombin. IV. The function of the fragment 2 region during activation in the presence of factor V. J Biol Chem. 1974 Dec 25;249(24):7791–7797. [PubMed] [Google Scholar]
- Esmon C. T., Owen W. G., Jackson C. M. The conversion of prothrombin to thrombin. II. Differentiation between thrombin- and factor Xa-catalyzed proteolyses. J Biol Chem. 1974 Jan 25;249(2):606–611. [PubMed] [Google Scholar]
- Gitel S. N., Owen W. G., Esmon C. T., Jackson C. M. A polypeptide region of bovine prothrombin specific for binding to phospholipids. Proc Natl Acad Sci U S A. 1973 May;70(5):1344–1348. doi: 10.1073/pnas.70.5.1344. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmsen H., Day H. J., Stormorken H. The blood platelet release reaction. Scand J Haematol Suppl. 1969;8:3–26. [PubMed] [Google Scholar]
- Owen W. G., Esmon C. T., Jackson C. M. The conversion of prothrombin to thrombin. I. Characterization of the reaction products formed during the activation of bovine prothrombin. J Biol Chem. 1974 Jan 25;249(2):594–605. [PubMed] [Google Scholar]
- Straughn W., 3rd, Wagner R. H. A simple method for preparing fibrinogen. Thromb Diath Haemorrh. 1966 Jul 31;16(1):198–206. [PubMed] [Google Scholar]
- Tollefsen D. M., Feagler J. R., Majerus P. W. Induction of the platelet release reaction by phytohemagglutinin. J Clin Invest. 1974 Jan;53(1):211–218. doi: 10.1172/JCI107540. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tollefsen D. M., Feagler J. R., Majerus P. W. The binding of thrombin to the surface of human platelets. J Biol Chem. 1974 Apr 25;249(8):2646–2651. [PubMed] [Google Scholar]
- Tollefsen D. M., Majerus P. W. Inhibition of human platelet aggregation by monovalent antifibrinogen antibody fragments. J Clin Invest. 1975 Jun;55(6):1259–1268. doi: 10.1172/JCI108045. [DOI] [PMC free article] [PubMed] [Google Scholar]