Table 1.

Primers correspond to VP7 and VP4 genes for rotavirus genotyping

Primer		Sequence (5′ – 3′)	Position	PCR product (bp)
G-typing (VP7) first amplification
	VP7-F	ATG TAT GGT ATT GAA TAT ACC AC	nt 51-71	881
	VP7-R	AAC TTG CCA CCA TTT TTT CC	nt 914-932
G-typing (VP7) second amplification
G1	aBT1	CAA GTA CTC AAA TCA ATG ATG G	nt 314-335	618
G2	aCT2	CAA TGA TAT TAA CAC ATT TTC TGT G	nt 411-435	521
G3	mG3	ACG AAC TCA ACA CGA GAG G	nt 250-269	682
G4	aDT4	CGT TTC TGG TGA GGA GTT G	nt 480-498	452
G8	aAT8	GTC ACA CCA TTT GTA AAT TCG	nt 178-198	754
G9	mG9	CTT GAT GTG ACT AYA AAT AC	nt 757-776	179
P-typing (VP4) first amplification
	VP4-F	TAT GCT CCA GTN AAT TGG	nt 132-149	663
	VP4-R	ATT GCA TTT CTT TCC ATA ATG	nt 775-795
P-typing (VP4) second amplification
P[4]	2T-1	CTA TTG TTA GAG GTT AGA GTC	nt 474–494	362
P[6]	3T-1	TGT TGA TTA GTT GGA TTC AA	nt 259–278	146
P[8]	1T-1	TCT ACT TGG ATA ACG TGC	nt 339 –356	224
P[9]	4T-1	TGA GAC ATG CAA TTG GAC	nt 385–402	270
P[10]	5T-1	ATC ATA GTT AGT AGT CGG	nt 575–594	462

References: [35].