Fig 3.

Apoptosis induced by Leu27-IGF-2. (A) Apoptotic cells were detected by TUNEL assay in NRVMs. Parallel cultured cells were stained with DAPI for nuclear labelling to count total cells per field of vision. Valinomycin treatment served as the positive control for apoptosis. (B) The percentage of apoptotic cells was calculated by the ratio of TUNEL-positive cells to total cells per field of vision. The data were obtained from six different visual fields of three independent experiments. Data are presented as mean ± S.D. **P < 0.01 and *P < 0.05 versus untreated control. ^#P < 0.05 versus Leu27-IGF-2–treated cells.