Fig 4.

ROS generation induced by Leu27-IGF-2. (A) ROS-positive cells were detected by fluorescent probe-Dihydroethidium (DHE) in NRVMs. Parallel cultured cells were stained with DAPI for nuclear labelling to count total cells per field of vision. Valinomycin treatment served as the positive control for ROS. (B) The percentage of ROS-positive cells was calculated by the ratio of ROS-positive cells to total cells per field of vision. The data were obtained from six different visual fields of three independent experiments. Data are presented as mean ± S.D. **P < 0.01 versus untreated control. ^##P < 0.01 versus Leu27-IGF-2–treated cells.