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(a) Normalized UMF signal of both donors and acceptors from one contrast agent (group #4 in Table 1) when ATTO 532-NHS:ATTO 647N-NHS:amine mole ratio is 0.3:0.03:1. (b) UMF modulation depth of both donor and acceptor as a function of ATTO 647N-NHS-to-amine mole ratio, where ATTO 532-NHS-to-amine mole ratio was fixed at 0.3.
