Fig. 3.

Expanded CD34+ keratinocytes in Schistosoma mansoni-infected mouse skin are located in the basal bulge of the hair follicle and are negative for mature keratins (Krt). (A) Cryosections from naïve (a) and infected (b; 24 h p.i.) pinnae labelled with antibodies specific for CD34. Image at 20× magnification (white arrows indicate hair follicles), and 63× magnification (white box, inset) showing individual hair follicles. (B) Individual and merged fluorescence channel views of an infected pinna cryosection labelled for CD34 and Krt 14, and counterstained with DAPI. Pinnae showing cells positive for (C) CD34 and Krt 6, and (D) CD34 and Krt 15. A and B are representative images from at least three independent experiments; C and D are representative images from at least two independent experiments. Scale bar = 50 μm.