Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Semin Nucl Med. 2015 Mar;45(2):163–176. doi: 10.1053/j.semnuclmed.2014.10.004

Figure 4.

Figure 4

3 additional 10 µm thick (adjacent) sections were collected from the same tumor. Upper left shows ‘flooded Hoechst’ where all tissue is stained with Hoechst 33342 to indicate position of nuclei. This shows areas of necrosis or missing tissue. On the upper right is shown the IHC image for EF5 binding, which is very comparable to that for the rinsed AR section (lower right panel, copied from Fig 3). On the lower left is shown the image resulting from the in vivo injection of Hoechst 33342 dye. The positions of the main vessels are easily seen. Note the central region of uniformly high binding. This is normal tissue stroma which one typically sees in orthotopic tumors – the vascular system of normal tissue is such that essentially every normal cell in the body is labelled by injected Hoechst 33342 (Koch and Evans, unpublished data).