Table 1.
Primer sequence used for real-time PCR using the Light Cycler
| Gene | Primer sequence | |
|---|---|---|
| IRAK-M | Forward, | GTACATCAGACAGGGGAAACTTT |
| Reverse, | GACATGAATCCAGGCCTCTC | |
| SIGIRR/ Tir8 | Forward, | CAGACCCATCTTCATCACCTTC |
| Reverse, | GCTGCACTTCTTTCCAAAAATC | |
| A20 | Forward, | TCCAGAACACCATTCCGTG |
| Reverse, | TGAGGTGCTTTGTGTGGTTC | |
| Tollip | Forward, | CTGATGCCAACAGTGTACCAG |
| Reverse, | ACATGTCCTGGATGGCTTTC | |
| ST2/ IL1RL1 | Forward, | TCAATAGGACTGGATATGCGA |
| Reverse, | GCCCTGTACCTTGATCCTTG | |
| MKP-1 | Forward, | CAACCACAAGGCAGACATCA |
| Reverse, | CTTCGCCTCTGCTTCACAA | |
| SOCS-1 | Forward, | AGAGCTTCGACTGCCTCTTC |
| Reverse, | AGGGGAAGGAGCTCAGGTAG | |
| SOCS-3 | Forward, | CAGTCTGGGACCAAGAACCT |
| Reverse, | GAGGAGGGYYCAGTAGGTGG | |
| β 2 -microglobulin | Forward, | CTCGCGCTACTCTCTCTTTCT |
| Reverse, | TGCTCCACTTTTTCAATTCTCT |
Irak-m: Interleukin (IL)-1 receptor (R) associated kinase-m, Sigirr: single immunoglobulin IL-1 related receptor (molecule), Tir8: Toll/IL-1R 8, Tollip: Toll interacting protein, IL1RL1: IL-1R like-1, MKP-1: Map kinase phosphatase-1, Socs: suppressor of cytokine signaling.