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. Author manuscript; available in PMC: 2015 Aug 5.
Published in final edited form as: Cell Metab. 2014 Jun 26;20(2):320–332. doi: 10.1016/j.cmet.2014.05.020

Figure 7. Shp2 is required for FGF15/19-stimulated FGFR4 activation.

Figure 7

(A–F) Serum-starved Hep3B cells were stimulated with 100 ng/ml hFGF19 as indicated. Immunoblotting was performed with indicated antibodies for total cell lysate (TCL) or immunoprecipitates. In E–F, the cells were treated with lenti-viruses expressing either scrambled (sh-scr) or Shp2-specific (sh-Shp2) shRNAs for 72 hrs before starvation.

(G) The same samples as in Figure 6D were blotted with antibodies to FGFR4 and β-actin.

(H) The same samples as in Figure 6C were immunoblotted for FGFR4 and Shp2.

(I) A model shows how Shp2 orchestrates BA and FGF15/19 signaling in control of BA biosynthesis.