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PF-4var/CXCL4L1 neutralizes permeability induced by VEGF in human retinal microvascular endothelial cells. HRMEC were seeded in transwells until confluency and were starved overnight in 1% serum-containing media. Cells were treated with vehicle, VEGF (50 ng/mL), or VEGF (50 ng/mL) plus 50 ng/mL PF-4var/CXCL4L1. FITC-conjugated dextran (1 mg/mL) was then added, and aliquots were assessed for fluorescence intensity using a fluorescence plate reader. n = 4 to 6, *P < 0.05.
