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Performance of the grid confocal microscope. Comparison of wide-field (a) and grid confocal (b) images for the same kidney sample as shown in Fig. 1c, d. The grid pattern is readily apparent when projected into a thin specimen (c) but is lost in the haze for an ∼50-μm-thick specimen (d). The CLSM (e) gives a much higher S/N, more accurate, and artifact-free image of the sample than the grid confocal (f). Scale bars, 20 μm.
