Skip to main content
. 2015 Mar 19;26(2):54–65. doi: 10.7171/jbt.15-2602-003

Figure 5.

Figure 5

Demonstration of the flexibility of the CLSM for scanning different sizes of the FOV with optimal resolution. Fixed HeLa cells labeled with DAPI (blue) and Alexa 488-H2AX (green), captured as a low-resolution overview image by scanning the entire FOV of a 63×/1.4 NA oil objective lens on a CLSM (a). A maximum-intensity projection of a 10-image z-stack of a zoomed-in area of the same sample as in (a) with an optimal pixel size of 0.1 μm (b). Maximum-intensity projection of a z-stack of a fixed rat brain section, imaged with a 20×/0.8 NA objective lens on a CLSM showing the complete SCN structure (c) labeled with DAPI (blue), arginine-vasopressin (green), and the proto-oncogene, C-Fos (red). Zoomed-in image of (c) showing that individual nuclei can be easily quantified for number and intensity from this large FOV high-resolution CLSM image (d). Scale bars, 10 μm (a and b) and 100 μm (c and d).