Figure 7.

Live-cell SDCM time series images. Successive frames of a time-lapse sequence show that microtubule dynamics in smooth muscle cells can be observed by imaging eGFP-tubulin using a 60×/1.4 NA oil objective on a Yokogawa SDCM (a–c). A zoomed-in time overlay image shows isosurfaces of the microtubules and changes in microtubule location and length (d). The differences in microtubule length between successive frames are modeled as cyan showing the location of the microtubules in frame (a). The green isosurfaces represent changes in microtubule position and length between frame (a) and frame (b). The red isosurfaces represent changes in the microtubule position and length from frame b to c. Isosurfaces were calculated and generated using Imaris software from Bitplane Incorporated (Zurich, Switzerland). The yellow arrowhead denotes the same location in each frame. The timescales in a–c are shown in seconds. Scale bars, 10 μm.