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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: Nature. 2014 Oct 12;515(7526):274–278. doi: 10.1038/nature13800

Figure 1. Generation of hNPCs with multiple FAD mutations.

Figure 1

a. Diagrams showing lentiviral IRES constructs. APPSL, APP with Swedish/London mutations; PS1ΔE9, PS1 with ΔE9 mutation; GFP, eGFP. b. Increased Aβ40 and 42 levels in 6-week differentiated FAD ReN cells. Aβ levels in conditioned media were normalized to total protein levels (*, p<0.05; **, p<0.01; ***, p<0.001; ANOVA followed by a post-hoc Dunnett test; n=3 per each sample). c. Aβ levels are dramatically decreased in FAD ReN cells after treatment with 1 μM BACE1 inhibitor IV or 3.7 nM Compound E (mean ± s.e.m; *, p<0.05; **, p<0.01; ***, p<0.001; ANOVA followed by a post-hoc Dunnett test; n=3 per each sample; n.d. not detected).