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. 2015 Mar 12;9:1511–1554. doi: 10.2147/DDDT.S75976

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© 2015 Zhou et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Sirt1 plays a role in PLB-induced apoptosis and autophagy in PC-3 and DU145 cells.

Notes: (A) PLB downregulating Sirt1 in PC-3 and DU145 cells as shown by Western blotting assay. Cells were treated with PLB at 0.1 μM, 1 μM, or 5 μM for 24 hours. (B) The bar graph shows the relative expression level of Sirt1 in PC-3 and DU145 cells. (C) Effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced apoptosis in PC-3 and DU145 cells. : (D) Bar graphs showing the effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced apoptosis in PC-3 and DU145 cells. (E) Representative flow cytometric dot plots showing the effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced autophagy in PC-3 and DU145 cells. (F) Bar graphs showing the effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL(25 μM) on PLB-induced autophagy in PC-3 and DU145 cells. (G) Effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced autophagy in PC-3 and DU145 cells as shown in confocal microscopic images (G) and bar graphs (H) using the autophagy-associated vacuole-specific fluorescence dye, Cyto-ID^®. Data are the mean ± SD of three independent experiments. *P<0.05; **P<0.01; and ***P<0.001 by one-way ANOVA.

Abbreviations: ANOVA, analysis of variance; DMSO, dimethylsulfoxide; PLB, plumbagin; STL, sirtinol; SD, standard deviation; WM, wortmannin; 7-AAD, 7-aminoactinomycin D; PE, phycoerythrin.

Sirt1 plays a role in PLB-induced apoptosis and autophagy in PC-3 and DU145 cells.

Notes: (A) PLB downregulating Sirt1 in PC-3 and DU145 cells as shown by Western blotting assay. Cells were treated with PLB at 0.1 μM, 1 μM, or 5 μM for 24 hours. (B) The bar graph shows the relative expression level of Sirt1 in PC-3 and DU145 cells. (C) Effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced apoptosis in PC-3 and DU145 cells. : (D) Bar graphs showing the effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced apoptosis in PC-3 and DU145 cells. (E) Representative flow cytometric dot plots showing the effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced autophagy in PC-3 and DU145 cells. (F) Bar graphs showing the effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL(25 μM) on PLB-induced autophagy in PC-3 and DU145 cells. (G) Effects of the Sirt1 inducer SRT (1 μM) and inhibitor STL (25 μM) on PLB-induced autophagy in PC-3 and DU145 cells as shown in confocal microscopic images (G) and bar graphs (H) using the autophagy-associated vacuole-specific fluorescence dye, Cyto-ID^®. Data are the mean ± SD of three independent experiments. *P<0.05; **P<0.01; and ***P<0.001 by one-way ANOVA.

Abbreviations: ANOVA, analysis of variance; DMSO, dimethylsulfoxide; PLB, plumbagin; STL, sirtinol; SD, standard deviation; WM, wortmannin; 7-AAD, 7-aminoactinomycin D; PE, phycoerythrin.