Table I. Primers and probes used for qRT-PCR.
| Receptor | Primer Sequence (5′ > 3′) | Probe1 (5′ > 3′) |
|---|---|---|
| αν |
F- TTCCCTTCCGGGTAGACG TA R- TGTGCAAAAATAATGCTCTTCGTAT |
TGCTGGATAAACACAAGCAAAGGGAGC |
| β3 |
F- TTTACCACTGATGCCAAGACTCA R- CCGTCATTAGGCTGGACAAT |
CATTGGACGGAAGGCTGGCAG |
| NTSR1 |
F- GGCGCCTCATGTTCTGCTA R- GTGCGTTGGTCACCATGTAGA |
ATGAGCAGTGGACTCCGTTCCTCTATGACTTCT |
| PSCA |
F- CAGGACTACTACGTG GGCAAGA R- CGCTGGCGTTGCACAA |
AACATCACGTGCTGTGACACCGA |
| PSMA |
F- GCTGATAAGCGAGGCATTAGT R- TGCGCGCCCTCCAA |
AGACTTTACCCCGCCGTGGTG |
1
All probes had a 5′-56-FAM fluorophore and a 36-TAMSp-3′ quencher