| Manufacturer |
Sysmex |
i+MED Laboratories Co. Ltd. |
| Instrumentation |
Haematology analyser |
Haematology analyser, magnet, rotator |
| Assay principle |
CD4 cell isolation using modified Dynal dynabeads, counting using haematology analyser with specific software |
Ferrous beads coated with MT4 mAb bind to CD4 T cells, which are then removed using magnet. Whole blood and CD4-deplete blood counted on haematology analyser. CD4 count and % calculated using established formulae |
| Parameters measured |
Absolute CD4 count and CD4% |
Absolute CD4 and CD4% |
| Specimen |
125 μl whole blood in EDTA |
400μl whole blood in EDTA |
| Throughput |
12 samples/hour |
No data available |
| Compatible with independent EQA/PT programmes? |
No EQA evaluation data available |
No EQA evaluation data available |
| Is there access to compatible full process QC reagent? |
No data available |
No data available |
| Maximum length of time from blood draw to testing |
24 hours at 4 or 20°C |
No data available |
| Can the test be performed on fixed/ stabilised blood |
No data available |
No data available |
| Complexity/training required |
Moderate complexity |
Moderate complexity |
| Environmental/energy issues |
Requires power for haematology analyser |
Requires power for haematology analyser and rotator |
| Robustness to heat/humidity |
No data available |
No data available |
