Fig 3. Coomassie-stained SDS-PAGE gels reveal that streptavidin-purified biotinylated proteins from different transgenes show differing protein patterns.

A. Shown are proteins purified from cells expressing biotin ligase alone (BL), biotin ligase fused to the N terminus of Ocln (BL-Ocln). B. Proteins purified from the N- and C- terminus of Ocln (BL-Ocln and Ocln-BL) with added biotin. C. Shown are proteins purified from cells expressing biotin ligase fused to the N terminus of Cldn4 (BL-Cldn4), with or without added biotin. The positions of the transgenes are marked with arrowheads. Triplicate samples gave very similar protein patterns.