Table 2. Detail of PCR primers used for Restriction fragment length polymorphism.

Gene	SNP name	alleles	Forward/Reverse primer sequence (5’-3’)	PCR product size	Restriction enzyme
TLR1	R80T	G>C	F-5’ TTCCTAAAGACCTATCCCAG3’	341 bp	Hinf1
			R-5’GAGCAATTGGCAGCACACTAG3’
TLR2	R753Q	G>A	F-5’ CATAAGCGGGACTTCATTCCTGCAA3’	372 bp	Pst1
			R-5’GATCCCAACTAGACAAGACTGGTCT3’
TLR3	S258G	A>G	F-5’GGGTCCCAGCCTTACAGAGA3’	341 bp	BsuRI
			R-5’CAATCTTGGGGAGTGAGGCA3’
TLR5	R392X	C>T	F-5’GGTAGCCTACATTGATTTGC3’	346 bp	Dde1
			F-5’GGATTCTCTGAAGGGGTTTAT3’
TLR5	N592S	A>G	F-5’GACTAAGCCTCAACTCCAACA3’	314 bp	Mun1
			R-5’GACTTCCTCTTCATCACAACC3;
TLR6	S249P	T>C	F-5’CTAGTTTATTCGCTATCCAAG3’	312 bp	Eco47I
			R-5’TTGTCAATGCTTTCAATGTCG3’

PCR = Polymerase chain reaction, TLR = Toll like receptor, bp = Base pairs, SNP = Single nucleotide polymorphism.