Table 5. Amplification of SNP markers based on the Brassica rapa genome to Brassica oleracea.
Primer | No. | PCR amplification | ||
---|---|---|---|---|
Amplification | No amplification | Rate (%) | ||
BRH a | 145 | 108 | 37 | 74.5 |
BRP b | 425 | 415 | 10 | 97.6 |
BRS c | 123 | 118 | 5 | 95.9 |
a. BRH: SNP marker developed from the Brassica rapa genome for high-resolution melt (HRM) analysis.
b. BRP: SNP marker based on the B. rapa genome that could be applied to B. oleracea.
c. BRS: New SNP marker derived from a BRP designed for HRM analysis.