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. 2015 Mar 19;10(3):e0118841. doi: 10.1371/journal.pone.0118841

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© 2015 Panda et al

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Fig 8 — Panel A—Western blot analysis of albumin using condition media obtained from different time points of hepatocytes culture using FBS-free William’s E media. Lane 1: pre-stained protein marker; Lane 2: fresh hepatocytes lysate; Lane 3: 1^st day CM; Lane 4: 3^rd day CM; Lane 5: 4^th day CM; Lane 6: 5^th day CM; Lane 7: 7^th day CM. Panel B—Control experiment. Lane 1: pre-stained protein marker; Lane 2: pure hepatocytes (positive control); Lane 3: condition media (test); Lane 4: FBS-free William’s E media (negative control).