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. 2015 Mar 19;10(3):e0120073. doi: 10.1371/journal.pone.0120073

Fig 1. While FGF1 enhances adipogenesis of hASCs in a dose-dependent manner, FGF2 displays biphasic effects on adipogenesis.

Fig 1

Human ASCs were pre-conditioned in the growth medium (GM) with various concentrations of ligands for 1 day, washed with PBS, treated in the differentiation medium (DM) for 6 days, and subjected to extraction of total RNA. Analyses of expression of aP2 (A), C/EBPα (B), and PPARγ (C) genes were carried out using real-time PCR with cyclophilin as an internal control. (D) Human ASCs were differentiated for 2 weeks and subjected to oil red O staining. (E) Human ASCs were pre-conditioned in the GM with the ligands for 1d, washed, treated with the DM for 16 h, and subjected to extraction of total RNA. Analysis of Spry4 was carried out using real-time PCR with cyclophilin as an internal control. Average values of each gene expression in the DM without ligands were calculated as 1 for statistical analysis. Results are presented as means ± SD.