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. 2015 Mar 19;10(3):e0120481. doi: 10.1371/journal.pone.0120481

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© 2015 Luz et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — A. RNA extraction from Stx2 IgG-producing hybridoma. Molecular marker (lane 1); Total RNA from anti-Stx2 IgG-producing hybridoma (mAb 2E11) (lane 2). B. scFv gene cloning confirmation. Molecular marker (lane 1); PCR product (lane 2). C. scFv fragment purification. Molecular weight ladder (lane 1); scFv elutions (lanes 2 and 3). D. Phage gene cloning confirmation. Molecular marker (lane 1); Double digestion (lane 2). Phage ELISA, results of independent experiments, performed in triplicate, are expressed as the means ± SEM * p < 0.05 compared with control. E. Electrophoretic analysis of Fab fragment purification. Molecular weight ladder (lane 1); Fab fragment purified (lane 2).