Fig 5. The N-terminal region of arfaptin-1 is required for localization to the Golgi.

(A) Schematic showing the arfaptin-1 N-terminal truncations and mutations used in this study. (B) HeLa cells were transfected with wild-type or truncated arfaptin-1b-myc constructs for 48 h. The cells were then stained with anti-myc and anti-Arl1 antibodies. Scale bars, 10 μm. Asterisks indicate cells expressing truncated or mutated arfaptin-1-myc constructs.