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. 2015 Mar 19;10(3):e0118743. doi: 10.1371/journal.pone.0118743

Fig 6. The effect of arfaptin-1b serine 132 phosphorylation on STxB endocytic transport.

Fig 6

HeLa cells were transfected with arfaptin-1a-S100A, arfaptin-1a-S100D, arfaptin-1b-S132A, or arfaptin-1b-S132D for 48 h. A STxB transport assay was conducted with incubation at 37°C for 30 min, followed by staining with anti-myc and anti-GM130 antibodies. Scale bars, 10 μm. Asterisks indicate the exogenous expression of myc-tagged arfaptin-1 constructs. The percentage of cells exhibiting inhibited STxB transport was quantified (n>50 for each experiment), and the data are presented as the means±SDs; p<0.05 indicates significance, as determined by one-way ANOVA.