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. 2015 Mar 19;10(3):e0119715. doi: 10.1371/journal.pone.0119715

Fig 4. Pharmacological inhibition of the intracellular calcium mobilization induced by LCFAs or GW9508 in CHO-K1/bFFAR1 cells.

Fig 4

CHO-K1 cells transfected with the bovine FFAR1 receptor were treated with solvent, the antagonist of the FFAR1 receptor, GW1100 (10 μM), for 15 min or the PLC inhibitor, U73122 (2 μM), for 3 min at 37°C, followed by solvent or 300 μM oleic acid (A and B), 100 μM linoleic acid (C and D) or 10 μM GW9508 (E and F). A representative experiment (upper graph) and area under the curve (AUC) between 60–150 s (bar graphs in lower graph) are shown. Arrows indicate the time of solvent or ligand addition. Mean ± SEM of 5 independent experiments. * p < 0.05. OA: oleic acid, LA: linoleic acid.