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. 2015 Mar 19;10(3):e0119715. doi: 10.1371/journal.pone.0119715

Fig 6. Effect of GW1100, U73122 or staurosporine on MMP-9 release induced by FFAR1 ligands in neutrophils.

Fig 6

Bovine neutrophils were incubated with 10 μM GW1100 for 15 min, 2 μM U73122 for 3 min or 2 μM staurosporine for 15 min, prior to the addition of 300 μM oleic acid (OA) (A, B and C), 100 μM linoleic acid (LA) (D, E and F) or 10 μM GW9508 (G, H and I) for 5 min at 37°C. PAF (100 nM) as positive control was used, and recombinant MMP-9 (rMMP-9) as size control was loaded in the gel (J). Supernatants were collected and analyzed by zymography. Bar graphs represent the mean ± SEM of 5 independent experiments. AU: arbitrary units. * p < 0.05, ** p < 0.01. OA: oleic acid, LA: linoleic acid.