Table 1. Bacterial strains and plasmids used in this study.
| Strains | Relevant genotype, phenotype or description a | Reference or source |
|---|---|---|
| Bacillus subtilis | ||
| PS832 | Wild-type prototroph | Lab strain |
| PS4389 | gerW1 (encodes 38 aa of GerW) Cmr | This work |
| PS4399 | gerW2 (encodes 6 aa of GerW) Cmr | This work |
| Bacillus megaterium | ||
| QM B1551 | Wild-type | Pat Vary |
| GC618 | gerU::pNFD13 Kmr | 10 |
| GC900 | gerW pHT-GerU* Znr Err | This work |
| GC918 | gerW::pNFD13 Kmr | This work |
| GC919 | gerD::pNFD13 Kmr | This work |
| Escherichia coli | ||
| DH5α | Competent cells | Lab strain |
| Plasmids | ||
| Modified pBluescript II KS | Ampr Cmr | Lab plasmid |
| pJCM 1 | gerW (-124 to +115) Ampr Cmr | This work |
| pJCM 2 | Plasmid with ΔgerW1 Ampr Cmr- | This work |
| pJCM 3 | Plasmid with ΔgerW2 Ampr Cmr | This work |
| pGEM-3Z | Ampr | Promega |
| P7Z6 | Ampr Znr | BGSC b |
| pUCTV2 | Ampr Tcr ts replication | 11 |
| pDONRtet | Gateway entry plasmid Tcr | 12 |
| pNFD13 | Vector to create lacZ fusions Kmr | 12 |
| pUC-ΔgerW::Zn | B. megaterium ΔgerW Ampr Tcr Znr | This work |
| pHT-GerU* | encodes GerU* GR genes Err | 13 |
aAbbreviations used are: ts, temperature sensitive; Ampr, ampicillin resistance (100 μg/ml); Cmr, chloramphenicol resistance (5 μg/ml); Err, erythromycin resistance (1 μg/ml); Kmr, kanamycin resistance (10 μg/ml); Tcr, tetracycline resistance (12.5 μg/ml); Znr, zeocin resistance (20 μg/ml).
bBacillus Genetic Stock Center