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. 2015 Mar 19;10(3):e0115997. doi: 10.1371/journal.pone.0115997

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© 2015 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A–D) Apoptosis of OPCs was analyzed labeling cells with DAPI (blue), GFP (green), and by the TUNEL reaction (red). Scale bar = 50 μm. (E–H) OPC differentiation into astrocytes was visualized by DAPI staining (blue) and GFP (green) and GFAP (red) immunolabeling. (I–L) OPC differentiation into neurons was visualized by DAPI staining (blue) and GFP (green) and NeuN (red) immunolabeling. (M–P) Myelin sheath formed by GFP⁺ OPCs was visualized by DAPI staining (blue), and GFP (green) and MBP (red) immunolabeling. Scale bar = 20 μm (E–P).