Fig 4. RnaC/S1022 is condition-dependently expressed.
Promoter activity of the PRnaC/S1022-gfp and Phag-gfp fusions in cells grown on LB (A), M9G (B), and M9S (C). Promoter activities were computed by subtraction of GFP level from the previous time-point as described by Botella et al. [38]. The experiment was performed three times in triplicate. Average data from triplicate measurements of one representative experiment are shown. The grey line indicates mean data and the black line a smoothed version of this mean. One in two time-points were plotted for the growth curve as open circles for the Phag-gfp strain and closed circles for the PRnaC/S1022-gfp strain. D) Representative FC results for PRnaC/S1022-gfp expression in mid-exponentially growing cells with or without a sigD deletion. The cells were grown in M9G. Wt, B. subtilis 168 not expressing GFP.