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. 2015 Mar 19;11(3):e1005046. doi: 10.1371/journal.pgen.1005046

Fig 7. AbrB levels determine the growth rate and growth yield on M9 minimal medium.

Fig 7

A) Representative Western blot data for AbrB production by the indicated strains grown on LB medium as a test for IPTG-dependent AbrB production from the amyE::abrB construct. At the lowest level of IPTG induction (0.01 mM), the AbrB production remained below the detection level. BdbD was used as a loading control. AbrB and BdbD were visualized with specific antibodies. B) Growth profiles on M9G determined for the strains from panel A. The abrB mutant is unable to grow on this medium, but its growth defect is rescued by IPTG-induced AbrB expression from the amyE::abrB construct. Averages of triplicate measurements from one representative 96-well plate experiment are shown.