Fig 3. Inhibition of biofilm formation with nisin A and nisin I4V peptides.
(A) Results of treatment of S. pseudintermedius DK729 with 1, 1/2, 1/4, 1/8 and 1/16X MIC of nisin A and nisin I4V peptides for 24 hrs prior to biofilm formation. The amount of biofilm was quantified by measuring the OD595 of crystal violet dissolved in acetic acid. The means and standard deviations of triplicate determinations are presented. Asterisks indicate statistically significant differences (Student’s t-test) between peptides used at similar concentration (** = p < 0.01) and (B) Growth curve analysis of strain S. pseudintermedius DK729 in 1X MIC peptides of nisin A (closed square), I4V (closed diamond) and no peptide (open circle).