Table 1. Oligonucleotides utilised in this study.
| Primer name | Sequence |
|---|---|
| NisI1degFOR | 5’ ATT ACA AGT NNK TCG CTA TGT ACA CCC GGT TGT AAA ACA 3’ |
| NisI1degREV | 5’ ACA TAG CGA MNN ACT TGT AAT GCG GGT TGA TGC ACC TGA 3’ |
| NisT2degFOR | 5’ CCA CGC ATT NNK AGT ATT TCG CTA TGT ACA CCC GGT TGT 3’ |
| NisT2degREV | 5’ CGA AAT ACT MNN AAT GCG GGT TGA TGC ACC TGA ATC TTT 3’ |
| NisI4degFOR | 5’ ATT ACA AGT NNK TCG CTA TGT ACA CCC GGT TGT AAA ACA 3’ |
| NisI4degREV | 5’ ACA TAG CGA MNN ACT TGT AAT GCG TGG TGA TGC ACC TGA 3’ |
| NisS5degFOR | 5’ ACA AGT ATT NNK CTA TGT ACA CCC GGT TGT AAA ACA GGA 3’ |
| NisS5degREV | 5’ TGT ACA TAG MNN AAT ACT TGT AAT GCG TGG TGA TGC ACC 3’ |
| NisL6degFOR | 5’ AGT ATT TCG NNK TGT ACA CCC GGT TGT AAA ACA GGA GCT 3’ |
| NisL6degREV | 5’ GGG TGT ACA MNN CGA AAT ACT TGT AAT GCG TGG TGA TGC 3’ |
| NisP9degFOR | 5’ CTA TGT ACA NNK GGT TGT AAA ACA GGA GCT CTG ATG GGT 3’ |
| NisP9degREV | 5’ TTT ACA ACC MNN TGT ACA TAG CGA AAT ACT TGT AAT GCG 3’ |
| NisG10degFOR | 5’ TGT ACA CCC NNK TGT AAA ACA GGA GCT CTG ATG GGT TGT 3’ |
| NisG10degREV | 5’ TGT TTT ACA MNN GGG TGT ACA TAG CGA AAT ACT TGT AAT 3’ |
| NisG14degFOR | 5’ TGT AAA ACA NNK GCT CTG ATG GGT TGT AAC ATG AAA ACA 3’ |
| NisG14degREV | 5’ CAT CAG AGC MNN TGT TTT ACA ACC GGG TGT ACA TAG CGA 3’ |
| NisA15degFOR | 5’ AAA ACA GGA NNK CTG ATG GGT TGT AAC ATG AAA ACA GCA 3’ |
| NisA15degREV | 5’ ACC CAT CAG MNN TCC TGT TTT ACA ACC GGG TGT ACA TAG 3’ |
| NisL16degFOR | 5’ ACA GGA GCT NNK ATG GGT TGT AAC ATG AAA ACA GCA ACT 3’ |
| NisL16degREV | 5’ ACA ACC CAT MNN CGA TCC TGT TTT ACA ACC GGG TGT ACA 3’ |
| NisM17degFOR | 5’ GGA GCT CTG NNK GGT TGT AAC ATG AAA ACA GCA ACT TGT3’ |
| NisM17degREV | 5’ GTT ACA ACC MNN CAG AGC TCC TGT TTT ACA ACC GGG TGT 3’ |
| NisG18degFOR | 5’ GCT CTG ATG NNK TGT AAC ATG AAA ACA GCA ACT TGT CAT 3’ |
| NisG18degREV | 5’ CAT GTT ACA MNN CAT CAG AGC TCC TGT TTT ACA ACC GGG 3’ |
| NisA24degFOR | 5’ ATG AAA ACA NNK ACT TGT CAT TGT AGT ATT CAC GTA AGC 3’ |
| NisA24degREV | 5’ ATG ACA AGT MNN TGT TTT CAT GTT ACA ACC CAT CAG AGC 3’ |
| NisH27degFOR | 5’ GCA ACT TGT NNK TGT AGT ATT CAC GTA AGC AAA TAA TCT 3’ |
| NisH27degREV | 5’AAT ACT ACA MNN ACA AGT TGC TGT TTT CAT GTT ACA ACC 3’ |
| NisI30degFOR | 5’ CAT TGT AGT NNK CAC GTA AGC AAA TAA TCT AGA GTCG AC 3’ |
| NisI30degREV | 5’ TTT GCT TAC GTG MNN ACT ACA ATG ACA AGT TGC TGT TTT 3’ |
| NisH31degFOR | 5’ TGT AGT ATT NNK GTA AGC AAA TAA TCT AGA GTC GAC CTG 3’ |
| NisH31degREV | 5’ TTT GCT TAC MNN AAT ACT ACA ATG ACA AGT TGC TGT TTT 3’ |
| NisV32degFOR | 5’ AGT ATT CAC NNK AGC AAA TAA TCT AGA GTC GAC CTG CAG 3’ |
| NisV32degREV | 5’ TTA TTT GCT MNN GTG AAT ACT ACA ATG ACA AGT TGC TGT 3’ |
| NisS33degFOR | 5’ ATT CAC GTA NNK AAA TAA TCT AGA GTC GAC CTG CAG CAA 3’ |
| NisS33degREV | 5’AGA TTA TTT MNN TAC GTG AAT ACT ACA ATG ACA AGT TGC 3’ |
| NisK34degFOR | 5’ CAC GTA AGC NNK TAA TCT AGA GTC GAC CTG CAG CAA TGG 3’ |
| NisK34degREV | 5’ TCT AGA TTA MNN GCT TAC GTG AAT ACT ACA ATG ACA AGT 3’ |
| pCI372FOR | 5’- CGGGAAGCTAGAGTAAGTAG -3' |
| pCI372Rev | 5’- ACCTCTCGGTTATGAGTTAG -3’ |
Emboldened sequences represent degenerate codons (N = A+C+G+T, K = G+T, M = A+C). Underlined sequence corresponds to plasmid (pCI372) DNA.