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. 2015 Mar 19;10(3):e0119617. doi: 10.1371/journal.pone.0119617

Fig 1. Feeder-layer independent induced pluripotent stem cells generation from HUVECs (HiPSCs).

Fig 1

(A) HUVECs were transduced with lentiviral vectors. Within 4 days (B) and a week (C) the endothelial cells were reduced in density but formed aggregates. (D) On day 16, immature colonies emerged. (E and F) Fully reprogrammed human embryonic stem cells (hESC)-like colonies were isolated after 21 days. Micrographs (G and I in low magnification) and (H and J in high magnification) show the morphology and quality of the colonies. Scale bars: A-F = 200 μm, G and I = 100 μm, H and J = 400 μm.