Table 2. Summary of cellular response to stimulants.
| Treatment | HFN | HiPSC-N | HFA | HiPSC-A | |
|---|---|---|---|---|---|
| Glutamate | Mean peak value | 450 ± 76.4 | 217.3 ± 3.5 | ||
| # of cells analyzed | 14 | 15 | |||
| # of replicates | 3 | 3 | |||
| Nicotine | Mean peak value | 330.3 ± 88.3 | 589.3 ± 28.8 | ||
| # of cells analyzed | 14 | 15 | |||
| # of replicates | 3 | 3 | |||
| Acetylcholine | Mean peak value | 1032 ± 212.7 | 1041.3 ± 129.5 | 681.3 ± 126.9 | 1764 ± 68.0 |
| # of cells analyzed | 14 | 15 | 15 | 15 | |
| # of replicates | 3 | 3 | 3 | 3 | |
| ATP | Mean peak value | 540.7 ± 36.4 | 800 ± 132.3 | ||
| # of cells analyzed | 15 | 15 | |||
| # of replicates | 3 | 3 | |||
Summary table showing the treatment of primary human fetal neurons (HFNs), HiPSC-derived neurons (HiPSC-Ns), primary human fetal astrocytes (HFAs), and HiPSC-derived astrocytes (HiPSC-As) with different stimulants (glutamate, nicotine and acetylcholine for neurons, and ATP and acetylcholine for astrocytes), and the responses of the cells to each treatment. The average peak value of the cell responses to each stimulant treatment is described as mean ± SEM of fluorescent intensity. The number of cells analyzed for each treatment in each group of cells and the number of replicates for each experiment are indicated.