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. 2015 Mar 19;10(3):e0120386. doi: 10.1371/journal.pone.0120386

Fig 2. Down-regulation of GPR120 in human eosinophils by ligand stimulation.

Fig 2

(A) After incubation with GW9508 (100 μM) for 10 min, nonpermeabilized eosinophils were stained with anti-GPR120 antibody (black histogram) or isotype-matched control (gray histogram) and then subjected to flow cytometric analysis. The histograms indicated monomodal expression of GPR120 and ligand-induced down-regulation. One of five experiments from different donors is shown. (B) After incubation with the indicated concentration of GW9508 for 10 or 30 min, the expression of GPR120 on the eosinophil surface was assessed as the MFI ratio. Data are expressed as the mean of five experiments ± SEM from different donors. *p<0.05 vs non-stimulated cells.