Figure 3.

In vivo inhibition of DsbB enzymes from gram-negative bacteria expressed in E. coli. E. coli dsbB mutant strains expressing β-Gal^dbs and dsbB genes from Salmonella typhimurium (St), Klebsiella pneumoniae (Kp), Vibrio cholerae (Vc), Haemophilus influenzae (Hi), Pseudomonas aeruginosa (Pa), Acinetobacter baumannii (Ab), Francisella tularensis (Ft) as well as two DsbB-homologs of P. aeruginosa (dsbH) and S. typhimurium (dsbI) and a non-homolog vkor from Mycobacterium tuberculosis (Mtb) were tested against pyridazinone-like compounds. Inhibition range from strong to weak is relative to each DsbB-expressing strain and was obtained by dividing the MIC of each compound between the lowest MIC observed for each particular strain. Results are the average of three independent experiments. Compounds that did not inhibit at the highest concentration tested are shown as black. Identity (%) compared to EcDsbB and protein length (amino acid) are indicated.