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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Invest Dermatol. 2014 Oct 22;135(4):1098–1107. doi: 10.1038/jid.2014.460

Figure 1. PKCε is a client protein of Hsp90β.

Figure 1

(a) UVR treatment increases the interaction of PKCε with Hsp90β. PKCε-overexpressing transgenic mice (line 224) (n=3) were exposed to UVR (4 kJ/m2) once. Mice were sacrificed 1 h or 2 h post UVR exposure. Epidermal protein extracts were prepared and pooled as described before (Aziz et al., 2007). 100 microgram of epidermal cell lysate protein was immunoprecipitated (IP) using PKCε antibody and immunoblotted with the Hsp90β antibody. (b) Co-localization of PKCε with Hsp90β. Co-localization of PKCε with Hsp90β in the indicated paraffin-fixed specimen was determined by double immunofluorescence as described (Aziz et al., 2007). The fluorescent pictures were captured through TRITC channel (red color), FITC channel (green color), and DAPI (Blue color) in a Nuance Fluorescent microscope. DAPI/PKCε/Hsp90β is the merge images of DAPI, FITC and TRITC channel. PKCε-overexpressing transgenic mouse skin harvested 24 h post UVR-exposure (1.8 kJ/m2) (bi), UVR induced SCC in SKH-1 hairless mice (bii) and Human SCC (biii). Shown is the representative picture selected from five different specimens.