Table 3.
Primary antibodies
| Antigen | Antibodies | Epitope or Immunogen | Dilution | Company; Catalog # (References) |
|---|---|---|---|---|
| rab5 | rabbit to human rab5A | C-terminus of human rab5A | 1:500 | Santa Cruz Biotechnology, Dallas, TX, # sc-309 (21, 22, 49, 50) |
| Cat D | mouse to human Cathepsin D [4G2] | 21-421 aa of E. coli Cathepsin D | 1:500 | LifeSpan BioSciences, Seattle, WA, #LS-B2931 |
| rabaptin5 | mouse to human rabaptin5 | 247-417 aa of human rabaptin5 | 1:500 | BD Biosciences, San Jose, CA, # 610676 (51, 52) |
| p62 | monoclonal to SQSTM1/p62 [EPR4844] | Human SQSTM1 sequence | 1:500 | Abcam, Cambridge, MA, # ab109012 (53) |
| traf6 | rabbit to traf6 | MATH domain of human traf6 | 1:200 | Millipore, Billerica, MA, # 06-1110 |
| pmTOR(s2481) | monoclonal to mTOR (phospho s2481) [EPR247(N)] | phospho-Ser 2481 of human mTOR | 1:500 | Abcam, # ab137133 |
| pmTOR(s2448) | monoclonal to mTOR (phospho s2448) [EPR246(2)] | phospho-Ser 2448 of human mTOR | 1:1000 | Abcam, # ab109268 (54, 55) |
| mTOR | rabbit to mTOR [7C10] | Ser2481 of human mTOR | 1:1000 | Cell Signaling Technology, Danvers, MA, # 2983S (56-58) |
| LilrB2 | mouse to LilrB2 | 22-599 aa of human LilrB2 | 1:500 | Abcam; # ab56696 (59) |
| β-tubulin | mouse to β-tubulin [KMX-1 clone] | tubulin from P. myxamoebae | 1:500 | Millipore, # MAB3408 (43, 60, 61) |
| β-tubulin | rabbit to β-tubulin | human β-tulubin | 1:500 | Cell Signaling Technology, #2146S (62) |
| Tau (AT8) | mouse monoclonal to phosphorylated tau | phospho-Ser202, phospho-Thr 205 | 1:500 | Fisher Scientific, Philadelphia, PA, # MN1020 (61, 63, 64) |
mTOR, mammalian target of rapamycin; pmTOR, phosphorylated mTOR; LilrB2, leukocyte immunoglobulin-like receptor B2.