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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Circ Heart Fail. 2014 Dec 30;8(2):352–361. doi: 10.1161/CIRCHEARTFAILURE.114.001893

Figure 1. Temporal appearance of M1-cells and M2-cells after Ang-II infusion.

Figure 1

A-C: Qualitative: After 1- and 7-day Ang-II infusion, WT heart sections were stained with fluorescence-labeled antibodies (blue = nuclear DAPI). Representative images are shown; they indicate an early infiltration of pro-collagen-type-I− M1-cells (CD86+), and a later presence of pro-collagen-type-I+ M2-cells (CD301+, CD206+). D-F: Quantitative: Isolated cells from WT and TNFR1-KO hearts were subjected to cytometry. “Live” cells were identified via calcein uptake. D1/E1/F1: In WT hearts, hematopoietic M1-cells (CD86+CD45+) appeared maximally at 3 days, whereas M2-cells (CD301+CD45+, CD206+CD45+) increased maximally by 7 days. In TNFR1-KO mice, M1-cells were equally upregulated, but M2-cells were fewer than in WT hearts. D2/E2/F2: CD86+ cells were collagen-type-I−, whereas CD301+ and CD206+ cells were collagen-type-I+. Statistics: (*) indicates a statistically significant difference between saline-treated (WT: n=8; TNFR1-KO: n=3) and 3-day (WT: n=6; TNFR1-KO: n=5) or 7-day (WT: n=7; TNFR1-KO: n=5-7) Ang-II-infused mice and (†) between 3-day and 7-day Ang-II-infused mice within the same genetic background (Kruskal-Wallis, Dunn's). NS: not significant.