Figure 5. Lineage tracing: TNFR1 signaling in fibroblast precursor uptake.
Chimeric mice (see Fig.4) were subjected to 7-day Ang-II infusion. Isolated cells were subjected to cytometry. A1/B1/C1: In chimeric TNFR1-KO/WT hearts, CD301+CD45+, CD206+CD45+, and CD34+CD45+ cells were increased compared to TNFR1-KO/TNFR1-KO hearts and were comparable to levels seen in WT/WT (n=3). A2/B2/C2: In chimeric TNFR1-KO/WT hearts, CD301+, CD206+, and CD34+ cells were also collagen-type-I+, similar to WT levels. Statistics: (*) indicates a statistically significant difference between TNFR1-KO/WT (n=7) and TNFR1-KO/TNFR1-KO (n=3) groups (Kruskal-Wallis, Dunn's). NS: not significant.
