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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Circ Heart Fail. 2014 Dec 30;8(2):352–361. doi: 10.1161/CIRCHEARTFAILURE.114.001893

Figure 6. In vitro differentiation of mouse monocytes-to-fibroblasts.

Figure 6

Mouse monocytes migrated through a confluent mouse endothelial layer in response to MCP-1. Successfully migrated cells were counted and characterized. A: Quantitative: Addition of Ang-II and TNF increased the amount of fibroblasts in WT cells, but not in TNFR1-KO. B-E: Qualitative: representative images of immunofluorescence staining. Cells after 4 days were spindle-shaped, CD206+, and pro-collagen-type-I+ (B); continued culturing resulted in CD301+pro-collagen-type-I+ cells (C). M2-cells (CD206+) also expressed TNFR1 (D). Cells after 1 day were small, round, and positive for M1 markers (CD86, iNOS; E). Statistics: For all groups n=6. (*) indicates a statistically significant difference between unstimulated WT and stimulated WT groups; (#) between stimulated WT and stimulated TNFR1-KO groups (one-way ANOVA, Tukey-Kramer). NS: not significant.